HERBICIDE TESTING, TOXICITY
AND EPA-REGISTRATION

This is a supplementary part of the Herbicidal Control page of this web site.

MAIN INDEX | Table of Aquatic Herbicides | Table of Relative Toxicity | Required EPA Studies |


Disastrous events of the past have caused the use of pesticides and other man-made chemicals to become a source of anxiety for the uninformed public. However, the U.S. Environmental Protection Agency has responded to previous mistakes by developing a program that requires stringent testing and strict regulation of herbicides and other pesticides prior to their commercial release for use in the environment.

We all want to know what chemicals we are buying and using, and we want to know their relative toxicity so we can know if these chemicals are dangerous or benign. EPA testing and registration enables the public to better understand the nature of the chemical being used and, by federal law, establishes minimum safety standards that safeguard public and environmental health.

Herbicide testing and registration is paid for by the chemical manufacturer and the studies are intended to determine:

  1. which plants are affected by the chemical
  2. environmental conditions the chemical must be applied in (i.e., sunny, cloudy, etc.)
  3. proper methods of application for the chemical
  4. relative toxicity to certain test plants and animals
  5. rate that the chemical breaks down and its longevity (persistence) in the soil or water
  6. presentation of the chemical's "use-label"

After EPA approval, the herbicide must also be registered with each state government. State registration may require additional testing for specific environmental or agricultural conditions. Registration in Florida is regulated by the Florida Department of Agriculture and Consumer Services.


REGISTERING AQUATIC HERBICIDES
Aquatic herbicides are a class of herbicides used to manage various aquatic weeds. Other states may require no additional testing or may not regulate herbicide use in aquatic situations at all. No matter the level of state regulation, federal law mandates that any herbicides used in, on, or over water must have an aquatic registration. Aquatic registration requires additional EPA testing. This is because aquatic herbicides are more prone to spread (via water flow) and can result in wider exposure to people and the environment.

Aquatic registration also takes special interest in the chemical's tolerance in fish and other aquatic species. Tolerance levels are determined through a series of tests that enables researchers to determine "no observable effect" levels in animal diets and quantification of residues in fish and water.

Acceptable toxicity level is defined as the level of pesticide present in fish or water in which a lifetime of human consumption is not likely to cause adverse health effects in humans.


The aquatic herbicides that are registered for use in Florida waters are listed with representative characteristics in the toxicity table shown below. These aquatic herbicides are approved for use by both the U.S. EPA and the Florida Department of Agriculture. (This table covers only those herbicides which are used in, on, or over water and does not include numerous ditchbank herbicides which may be used adjacent to, but not in water.)

In this table,

HERBICIDEUSE RATEACUTE TOXICITY (Rat)
(Bluegill)
HALF-LIFEELIMINATION PROCESSES

Copper

1 mg/l

470 mg/kg

1-12 mg/l (hardness)

2-8 days

adsorption
(sticks to soil)

precipitation
(falls to bottom)

Diquat

0.1-0.5 mg/l

230 mg/kg (Rat)

67 mg/l (Bluegill)

1-4 days (w)2

100-1000 days2

photolysis
(degraded by sunlight)

microbial
(used by microbes)

adsorption
(to soil)

Endothall

2-4 mg/l

51 mg/kg (acid)

240 mg/l (K-salt)

1-7 days

microbial
(used by microbes)

Fluridone

10-20 micrograms/l

> 10,000 mg/kg

14 mg/l

20-80 days

photolysis
(sunlight)

microbial
(microbes)

Glyphosate

0.5-0.5mg/l

> 5000 mg/kg
>1000 mg/l

5-10 days

40-50 days

microbial
(microbes)

2, 4-D

to 1.0 mg/l

764 mg/kg

263 mg/l

2-6 days

microbial
(microbes)

Triclopyr
(Amine)
(imazapyr)

1.0 mg/l

713 mg/kg (2600)

148 mg/l (891)

12-72 hrs

photolysis
(sunlight)


HOW TOXIC IS TOXIC
The following chart compares the toxicity
of common aquatic herbicides to the toxicity of certain everyday chemicals. This chart is included so that readers can begin to gauge the relative risks of aquatic herbicide use. Bold lettering indicates an aquatic herbicide. (Chart prepared by V. Ramey, based on published studies.)

TOXICITY OF AQUATIC HERBICIDES RELATIVE TO CERTAIN EVERYDAY CHEMICALS

CHEMICAL LETHAL DOSE 50
(the dose required to kill 50% of the test animals)
milligrams of chemical per
kilogram of animal weight
TOXICITY
(from least toxic to more toxic)

Tetramethrin (Hot Shot-Insecticide)

20,000

*

Fluridone (Herbicide)

10,000

**

Oryzalin (Herbicide)

10,000

**

Fluometuron (Herbicide)

8,000

**

Glyphosate (Herbicide)

5,600

***

Atrazine (Herbicide)

3,080

***

Table Salt

3,000

***

Malathion (Insecticide)

2,800

***

Metribuzin (Herbicide)

2,200

***

Aspirin

1,000

****

2,4-D (Herbicide)

500

*****

Chlordane (Insecticide)

350

*****

Copper Sulfate (Algicide)

300

*****

Diquat (Herbicide)

230

*****

DDT (Insecticide)

215

*****

Endothall (Herbicide)

206

*****

Caffeine (found in coffee, cola, tea)

192

******

Chlorpyrifos
(Rid-A-Bug, flea collars)

163

******

Heptachlor (Insecticide)

105

******

Lindane (Insecticide)

88

******

Endosulphan (Insecticide)

64

******

Nicotine (found in tobacco)

53

*******

Dieldrin (Insecticide)

45

*******

Aldrin (Insecticide)

37.5

*******

Sodium Fluoride (toothpaste ingredient)

31

*******

Sodium Cyanide

6.4

********

Amatoxin (found in mushrooms)

0.15

********

Dioxin (TCDD)

0.0006

*********

Aflatoxin (found in peanut butter, eggs, corn)

0.0006

*********


A LITTLE DEEPER
A pesticide cannot be legally used
if it has not been registered with EPA's Office of Pesticide Programs.

EPA registration of pesticides intends to promote the safety and well-being of public health and the ecosystem. In order to release a pesticide for legal use, the EPA evaluates the chemicals in the pesticide in relation to the location or crop on which it is to be used; the amount, frequency and timing of application; and storage and disposal methods. Pesticide evaluation also seeks to ensure that the pesticide will not have unreasonable adverse effects on humans, the ecosystems, and non-target species of plants and animals.

The following assessments are used during the chemical evaluation process:


EPA Pesticide Registration Program
EPA Pesticide Regulation
EPA Ecotoxicology Database - find toxicity information on individual chemicals
EPA Pesticide Registration Notices
EPA Reregistration of Pesticides registered before November 1984


The Creators

This page was authored by Sarah Cervone with research assistance from Rebecca Hassell.
This page was designed and is managed by Rebecca Hassell.
Photography and graphics are by Ann Murray and Vic Ramey.
Vic Ramey is the editor.

DEP review by Jeff Schardt and Judy Ludlow.

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A collaboration of the Center for Aquatic and Invasive Plants, University of Florida, and the Invasive Plant Management Section of the Florida Fish and Wildlife Conservation Commission.


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